Composite

Part:BBa_K5425004:Design

Designed by: Sampoorn Pandey   Group: iGEM24_IISER-Kolkata   (2024-09-21)


The population control module is a carefully engineered circuit that regulates cell density through


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3784
    Illegal EcoRI site found at 3368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3368
    Illegal SpeI site found at 3785
    Illegal PstI site found at 3799
    Illegal NotI site found at 3374
    Illegal NotI site found at 3792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3785
    Illegal EcoRI site found at 3368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3368
    Illegal SpeI site found at 3785
    Illegal PstI site found at 3799
    Illegal AgeI site found at 2493
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All design of this part started with the modelling of this part using deterministic and stochastic methods.Every part was chosen in a regime which could impart oscillations.


Source

Most of the sequences taken to make this parts were available on the registry.A new basic part which was designed by us which included the anti sense mRNA against the dnaA protein was taken from bl21(de3) coli.

References